Test Code: 4531CPT: 83540
|Use||Used in conjunction with TIBC and Transferrin to assess how much iron is in the blood and to diagnose iron deficiency or overload.|
|Clinical Utility||Low result indicates Iron Deficiency and/or Chronic Illness. High results indicate Hemochromatosis or Hemolytic Anemia or Sideroblastic Anemia or Iron Poisoning|
|Intended Patient Population||18+ and Older Adult Males & Females|
|Tube||Red, Green, Tiger|
|Volume||4mL Whole Blood (1mL Serum)|
|Min Sample Volume||0.5 mLs|
|Reference Ranges||M & F ≥ 18 yrs old; 50-212 ug/dL|
|Analytical Measurement Range||10-1000 ug/dL|
|Critical Values||> 500 ug/dL|
|Test Methodology||Tripyridyltriazine (TPTZ)|
|Test Turnaround Time||2 Days|
|Limitations||1. EDTA, sodium citrate, and potassium oxalate are known to interfere with this method.
2. Samples showing evidence of hemolysis should not be used. Hemolysis may cause falsely elevated results.
3. Ingestion of oral contraceptives will elevate iron and/or total iron binding capacity values.2
4. Iron-dextran administration can cause elevations in total serum iron with this methodology.
5. Use of this assay is not recommended for patients undergoing treatment with deferoxamine (e.g., Desferal) or other
6. Ingestion of iron (including iron-forti ed vitamins or supplements) may cause transient elevated iron levels.3
7. Some gadolinium magnetic resonance contrast agents such as Omniscan, Optimark, and Magnevist may interfere
with this method.4
|Specimen Stability||7 Days RF|
|Reject Criteria||Gross Hemolysis, Gross Lipemia|
|Laboratory Developed Test (LDT)||Yes|
|CMS Guidance||NCD 190.18|
|References||1. Tietz, N. W., Textbook of Clinical Chemistry, W. B. Saunders Company, Philadelphia, PA (1986).
2. Weissman, M., Pileggi, V. J., Clinical Chemistry: Principles and Techniques, Harper and Row Publishers, Hagerstown, MD (1974).
3. Burtis, C. A., Ashwood, E. R., Tietz Textbook of Clinical Chemistry 3rd Edition, pp 1701, W. B. Saunders, Philadelphia, PA (1999).
4. Kerry, A.S. et al. "Gadolinium Magnetic Resonance Contrast Agents Produce Analytic Interference in Multiple Serum Assays." Am J Clin Pathol 121:282-92 (2004).