Test Code: 4019CPT: 82746
|Use||Folate is an essential vitamin vital to normal cell growth and DNA synthesis. It is present in a wide variety of foods such
as dark, leafy vegetables, citrus fruits, yeast, beans, eggs, and milk. It is absorbed by the small intestine and stored in
the liver. A folate deficiency can lead to megaloblastic anemia and ultimately to severe neurological problems.1,2,3 Used in conjuction with Vit B12 to help diagnose certain anemias that affect the absorption of B12.
|Clinical Utility||Folate deficiency can be caused by insufficient dietary intake, malabsorption or excessive folate utilization. Excessive utilization occurs very commonly during pregnancy. Alcoholism, hepatitis, or other liver-damaging diseases can also cause excessive folate utilization.1,2,3 Folate levels in both serum and red blood cells are used to assess folate status. The serum folate level is an indicator of recent folate intake. Red blood cell (RBC) folate is the best indicator of long term folate stores. A low RBC folate value can indicate a prolonged folate deficiency.
Folate and vitamin B12 are linked by the reaction pathway for methionine synthesis. A deficiency in either leads to a disruption of this pathway and to similar clinical symptoms.1,3 Another consequence of this common metabolic pathway is that a B12 deficiency disrupts the uptake of folate into red blood cells. This leads to a low RBC folate value even with adequate folate intake. For the above reasons, it is often necessary to measure both vitamins in a clinical workup. The treatment depends on which vitamin is deficient.
|Intended Patient Population||18+ and Older Adult Males & Females|
|Patient Preparation||None Specified|
|Tube||Red, Green, Tiger|
|Volume||4mL Whole Blood (1mL Serum/Plasma)|
|Min Sample Volume||0.5 mLs|
|Reference Ranges||M & F ≥ 18 yrs old; 3.56-20.00 ng/mL|
|Analytical Measurement Range||0.50 -20.00 ng/mL|
|Test Methodology||Chemiluminescent Immunoassay|
|Test Turnaround Time||2 Days|
|Specimen Stability||7 Days RF|
|Reject Criteria||Gross Hemolysis, Excess Light Exposure|
|Laboratory Developed Test (LDT)||Yes|
|References||1. Donnelly, J. Folic Acid. Critical Reviews in Clinical Laboratory Sciences. 2001; 38(3): 183-223.
2. Snow CF. Laboratory diagnosis of vitamin B12 and folate deficiency: A guide for the primary care physician. Arch
Intern Med. 1999;159:1289-1298.
3. Fairbanks VF, Klee GC. Biochemical aspects of hematology. In Burtis CA, Ashwood ER (eds): Tietz textbook of clinical chemistry, 3rd edition, pp 1642-1710, Philadelphia, WB Saunders, 1999.