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Insource Diagnostics


Alternative Names
  • Cholesterol
  • Total
  • Serum
  • Lipid Screen (Cholesterol, Trig, Chol HDL, Calculated LDL)

Test Code: 4527

CPT: 82465
Tests Included Cholesterol
Use Measurements of cholesterol are used primarily in the diagnosis and treatment of disorders involving excess cholesterol in the blood and lipid/lipoprotein metabolism disorders.
Clinical Utility Total serum cholesterol analysis has proven useful in the diagnosis of hyperlipoproteinemia, atherosclerosis, hepatic and thyroid diseases. Total and HDL cholesterols, in conjunction with a triglyceride determination, provide valuable information for the prediction of coronary heart disease (CHD).3
Intended Patient Population 18+ and Older Adult Males & Females
Patient Preparation None Specified
Sample Serum, Plasma
Tube Red, Green, Tiger 
Volume 4mL Whole Blood (1mL Serum/Plasma)
Min Sample Volume 0.1 mLs
Reference Ranges M & F ≥ 18 yrs old; < 200 mg/dL
Analytical Measurement Range 35-715 mg/dL
Units mg/dL
Test Methodology Quantitative Enzymatic
Test Turnaround Time 1 Day
Limitations N-Acetyl Cysteine (NAC), when administered in therapeutic concentrations (for the treatment of acetaminophen overdose), has been...determined to interfere with assays for... Cholesterol, Uric Acid" where "NAC interference may lead to falsely low results." The NAC interference should be insignificant by 12 hours after completion of the initial loading dose of an IV infusion treatment regimen consisting of an initial loading dose of 150 mg/kg administered over 1 hour, a second dose of 50 mg/kg administered over 4 hours and a third dose of 100 mg/kg administered over 16 hours.
Shipping Requirements Refrigerated
Specimen Stability 7 Days RT
7 Days RF
Reject Criteria Hemolysis, Mild Icterus
Laboratory Developed Test (LDT) Yes
CMS Guidance NCD 190.23
References 1. Tietz Textbook of Clinical Chemistry. Edited by CA Burtis, ER Ashwood. Philadelphia, WB Saunders Company, 1999
2. National Institute of Health (NIH) Publication: Second Report of the Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults. NIH Publication 93-3096. 1993 Sep;93:3096
3.Rifkind BM, Segal P. Lipid research clinics program reference values for hyperlipidemia and hypolipidemia. JAMA. 1983 Oct 14; 250(14):1869-1872