Test Code: 4503CPT: 84450
|Use||Measurement of AST is indicated in the diagnosis, differentiation and monitoring of hepatobiliary disease, myocardial infarction, accute liver cell damage, and skeletal muscle damage. AST may also be useful in identifying liver diseses including cirrhosis, viral hepatitis and carbon tetrachloride poisoning|
|Clinical Utility||AST occurs in a wide variety of tissues including liver, heart, skeletal muscle, brain, kidneys, lungs, pancreas, erythrocytes and leucocytes. The highest concentrations are obsrved in liver, heart skeletal muscle, and kidney.
AST levels may be increased in viral hepatitis and liver disease associated with hepatic necrosis, with 20 to 50 fold elevations frequently encountered. Increased AST levels may be detected in cirrhosis, extrahepatic cholestasis, progressive muscular dystrophy, dermatomyositis, acute pancreatitis, haemolytic disease, gangrene, crushed muscle injuries and pulmonary emboli. Slight or moderate increases in AST levels may also be observed after ingestion of alcohol, or administration of drugs including penicillin, [rpgesterone, anabolic-androgenic steroids, salicylates or opiates.
Where recent myocardial infarction is suspected, AST has a diagnostic sensitivity of 96%, with a diagnostic sensitivity of 86% at 12 hours after onset of chest pain
|Intended Patient Population||18+ and Older Adult Males & Females|
|Patient Preparation||None Specified|
|Tube||Red, Green, Tiger|
|Volume||4mL Whole Blood (1mL Serum/Plasma)|
|Min Sample Volume||0.1 mLs|
|Reference Ranges||M ≥ 18 yrs old; < 50 U/L
F ≥ 18 yrs old; < 35 U/L
|Analytical Measurement Range||4-734 U/L|
|Test Methodology||Quantitative Enzymatic|
|Test Turnaround Time||1 Day|
|Limitations||Samples with extremely high enzyme activity (>12,000 IU/L or >200.04 µkat/L) may cause abnormally low readings|
|Specimen Stability||5 Days RT
7 Days RF
|Reject Criteria||Mild Hemolysis|
|Laboratory Developed Test (LDT)||Yes|
|References||1. Tietz Textbook of Clinical Chemistry. Edited by CA Burtis, ER Ashwood. Philadelphia, WB Saunders Company, 1999
2. Rej R. Aminotransferase in disease. Clin Lab Med. 1989 Dec; 9(4):667-687.